METHODS AND SYSTEM FOR SPECTRAL IMAGING OF DENSITY, ANISTROPY, AND ORIENTATION
Researchers at the Chan Zuckerberg Biohub have developed an imaging module and computational algorithms for high-speed spectral quantitative imaging of density and anisotropy in fluorescence and label-free contrasts.
Microscopy is central to biological research and has enabled scientist to study the structure and dynamics of cells and their components within. The spatial and angular organization of biological macromolecules is a key determinant, as well as informative readout, of their function. Correlative imaging of the dynamic spatio-angular architecture of cells and organelles is valuable, but requires fast polarization-, depth-, and wavelength-diverse measurements of intrinsic optical properties and fluorescent labels.
Stage of Research
The inventors have developed a multimodal instant polarization microscope that combines a broadband polarization-resolved detector, automation, and reconstruction algorithms to enable label-free imaging of phase, retardance, and orientation, multiplexed with fluorescence imaging of concentration, anisotropy, and orientation of molecules at diffraction-limited resolution at high speed. The images are calibrated, background corrected, denoised, and reconstructed to provide high resolution imaging of spatio-angular architecture of organelles, cells and tissues. The authors demonstrate the utility for acquiring fast measurements of density and anisotropy in dynamic samples with multiple fluorophores and label-free imaging at different wavelengths.
Applications
- Polarization-, depth-, and wavelength-diverse imaging for quantitative measurement of density and anisotropy, with or without fluorescent labels
- Broad commercial applications, including material inspection and digital pathology
Advantages
- New calibration method and data denoising improve reconstruction of anisotropy
- Multiplexed measurements can be made across the visible spectrum and at high temporal and spatial resolution
- Imaging module is easily integrated into many commercially available microscopes
Stage of Development
Research – in vitro
Publications
Ivanov IE, Yeh L-H, et al., Correlative imaging of the spatio-angular dynamics of biological systems with multimodal instant polarization microscope. Biomedical Optics Express. 2022. Doi: 10.1364/BOE.455770
PCT Publication No. WO2021242855
Related Web Links
https://www.czbiohub.org/comp-micro/
Keywords
Biomolecules, microscopy, optical imaging, polarization, density, anistropy
Technology Reference
CZB-152
