http://czbiohub.technologypublisher.comBe the first to know about the latest inventions and technologies available from CZ Biohuben-USTue, 14 Apr 2026 00:26:16 GMTThu, 23 Oct 2025 13:21:30 GMThttp://blogs.law.harvard.edu/tech/rssBonnevie.Bernardino@czbiohub.orgCopyright 2026, CZ BiohubCZB-327Shttp://czbiohub.technologypublisher.com/technology/58938Thu, 23 Oct 2025 13:21:30 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58938CZB-322Bhttp://czbiohub.technologypublisher.com/technology/58937Thu, 23 Oct 2025 13:08:14 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58937CZB-320Fhttp://czbiohub.technologypublisher.com/technology/58936Thu, 23 Oct 2025 13:01:47 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58936CZB-318Shttp://czbiohub.technologypublisher.com/technology/58935Thu, 23 Oct 2025 12:58:10 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58935CZB-317Bhttp://czbiohub.technologypublisher.com/technology/58933Researchers at UC Berkeley have developed novel strategies for the delivery of CRISPR gene editing components. While CRISPR-based gene editing holds great promise for treating genetic diseases, it often involves DNA breaks or base changes that can cause off-target effects, cell damage, or unpredictable outcomes. Additionally, these changes are often permanent and may lead to harmful consequences. Epigenetic editing offers a safer alternative by enabling reversible, long-term regulation of gene expression without altering the DNA sequence itself. This approach also allows for more versatile ed...Thu, 23 Oct 2025 11:22:03 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58933CZB-316Fhttp://czbiohub.technologypublisher.com/technology/58932Thu, 23 Oct 2025 11:16:43 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58932CZB-315Fhttp://czbiohub.technologypublisher.com/technology/58931Researchers at CZ Biohub SF and UCSF have developed a novel diagnostic application for Chagas disease using a selective antigen. Chagas disease is an infection caused by the parasite Trypanosoma cruzi (T. cruzi). This disease is primarily endemic to Latin America but increasingly recognized in the United States, particularly among Latinx immigrant populations. Chagas disease can persist chronically and asymptomatically for decades, during which the parasite resides intracellularly, ultimately leading to irreversible cardiac and gastrointestinal damage. Serological detection of anti-T. cruzi a...Thu, 23 Oct 2025 11:00:09 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58931CZB-312Shttp://czbiohub.technologypublisher.com/technology/58930Thu, 23 Oct 2025 10:58:31 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58930CZB-303Fhttp://czbiohub.technologypublisher.com/technology/58927Thu, 23 Oct 2025 09:33:23 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/58927CZB-314Bhttp://czbiohub.technologypublisher.com/technology/55536VOLUME IMAGING OF BIOLOGICAL SPECIMENS BY PHOTOCHEMICAL SECTIONING Researchers at UC Berkeley and University of Illinois, Chicago have developed a nanoscale volume fluorescence imaging method that enables high-resolution imaging of large tissue volumes. Hydrogel-based tissue clearing and expansion techniques have improved the ability to study cellular structures and molecular details within intact tissues at resolutions beyond the diffraction limit of light. However, current fluorescence imaging methods face a trade-off between imaging depth and resolution, particularly when applied to lar...Wed, 06 Nov 2024 20:22:43 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/55536CZB-311Shttp://czbiohub.technologypublisher.com/technology/555353D PRINTING OF ORGANOID SLURRIES Researchers at Stanford have developed methods for 3D printing and post-biomanufacturing of organoid slurries in a high yield stress matrix. Biomanufactured tissues and organs on demand has been a long-standing challenge in the field of tissue engineering. Despite the advancements allowed by 3D bioprinting, current techniques remain sub-optimal for both printing dynamics and post-biomanufacturing maturation of the cells in the printing structure. Stage of Research The inventors have developed methods for the biomanufacturing of large-scale tissue construct...Wed, 06 Nov 2024 20:19:27 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/55535CZB-302Shttp://czbiohub.technologypublisher.com/technology/55534GENETICALLY ENCODED LYSOSOME TARGETING CHIMERAS FOR CELL-MEDIATED DELIVERY Researchers at Stanford have developed fully genetically-encodable lysosome-targeting chimeras which allow for the targeted delivery of various proteins into receiver cells. Lysosome-targeted degradation is an emerging therapeutic modality that facilitates the degradation of membrane and soluble extracellular proteins. Compared to traditional therapeutic modalities, such as small molecule or antibody-based inhibitors, targeted protein degradation offers increased potential potency and broadens the druggable proteome....Wed, 06 Nov 2024 20:14:32 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/55534CZB-292Shttp://czbiohub.technologypublisher.com/technology/55533COMPOSITIONS AND METHODS RELATED TO CORONAVIRUS THERAPIES Researchers at Stanford have developed fusion proteins, containing ACE2 domain linked to a fragment of non-neutralizing anti-SARS-CoV-2 spike protein antibody, with a greater breadth of protection than previously described similar fusion proteins. Coronaviruses are a large family of viruses that cause illness ranging from the common cold to more severe diseases, including Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). Coronaviruses are zoonotic, meaning they can be transmitted between humans and...Wed, 06 Nov 2024 20:10:47 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/55533CZB-310Shttp://czbiohub.technologypublisher.com/technology/55192Tue, 24 Sep 2024 16:23:57 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/55192CZB-309Shttp://czbiohub.technologypublisher.com/technology/55191Tue, 24 Sep 2024 16:21:02 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/55191CZB-306Shttp://czbiohub.technologypublisher.com/technology/55190Tue, 24 Sep 2024 16:18:02 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/55190CZB-293Shttp://czbiohub.technologypublisher.com/technology/55189Tue, 24 Sep 2024 16:13:21 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/55189CZB-300Shttp://czbiohub.technologypublisher.com/technology/54361Thu, 06 Jun 2024 10:55:34 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/54361CZB-299Shttp://czbiohub.technologypublisher.com/technology/54360Thu, 06 Jun 2024 10:50:24 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/54360CZB-296Fhttp://czbiohub.technologypublisher.com/technology/54358Thu, 06 Jun 2024 10:46:50 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/54358CZB-291Shttp://czbiohub.technologypublisher.com/technology/53993Mon, 22 Apr 2024 18:27:37 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/53993CZB-286Bhttp://czbiohub.technologypublisher.com/technology/53992Mon, 22 Apr 2024 18:01:44 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/53992CZB-281Shttp://czbiohub.technologypublisher.com/technology/53991Mon, 22 Apr 2024 17:57:51 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/53991CZB-270Bhttp://czbiohub.technologypublisher.com/technology/53990Mon, 22 Apr 2024 17:53:32 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/53990CZB-298Shttp://czbiohub.technologypublisher.com/technology/53347Wed, 14 Feb 2024 17:50:10 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/53347CZB-294Shttp://czbiohub.technologypublisher.com/technology/53346Wed, 14 Feb 2024 17:47:36 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/53346CZB-287Bhttp://czbiohub.technologypublisher.com/technology/53345Wed, 14 Feb 2024 17:45:30 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/53345CZB-282Shttp://czbiohub.technologypublisher.com/technology/53344Wed, 14 Feb 2024 17:43:13 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/53344CZB-285Shttp://czbiohub.technologypublisher.com/technology/52880Tue, 05 Dec 2023 14:15:12 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52880CZB-284Fhttp://czbiohub.technologypublisher.com/technology/52879Tue, 05 Dec 2023 14:12:15 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52879CZB-280Shttp://czbiohub.technologypublisher.com/technology/52878Tue, 05 Dec 2023 14:09:46 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52878CZB-279Fhttp://czbiohub.technologypublisher.com/technology/52877Tue, 05 Dec 2023 14:07:16 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52877CZB-277Bhttp://czbiohub.technologypublisher.com/technology/52668Thu, 26 Oct 2023 11:37:50 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52668CZB-276Shttp://czbiohub.technologypublisher.com/technology/52666Thu, 26 Oct 2023 11:32:55 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52666CZB-275Fhttp://czbiohub.technologypublisher.com/technology/52665Thu, 26 Oct 2023 11:30:49 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52665CZB-271Fhttp://czbiohub.technologypublisher.com/technology/52664Thu, 26 Oct 2023 11:25:55 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52664CZB-267Shttp://czbiohub.technologypublisher.com/technology/52553Fri, 06 Oct 2023 10:13:17 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52553CZB-199Shttp://czbiohub.technologypublisher.com/technology/52551Fri, 06 Oct 2023 10:08:36 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52551CZB-122Shttp://czbiohub.technologypublisher.com/technology/52550Fri, 06 Oct 2023 10:06:12 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52550CZB-264Shttp://czbiohub.technologypublisher.com/technology/52427Thu, 14 Sep 2023 12:32:08 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52427CZB-260Shttp://czbiohub.technologypublisher.com/technology/52426Thu, 14 Sep 2023 12:27:29 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52426CZB-259Shttp://czbiohub.technologypublisher.com/technology/52425Thu, 14 Sep 2023 12:23:35 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52425CZB-257Shttp://czbiohub.technologypublisher.com/technology/52423Thu, 14 Sep 2023 12:19:09 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52423CZB-256Fhttp://czbiohub.technologypublisher.com/technology/52422Thu, 14 Sep 2023 12:07:54 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52422CZB-245Shttp://czbiohub.technologypublisher.com/technology/52421Thu, 14 Sep 2023 12:06:02 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52421CZB-237Bhttp://czbiohub.technologypublisher.com/technology/52420Thu, 14 Sep 2023 12:03:33 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52420CZB-236Fhttp://czbiohub.technologypublisher.com/technology/52419INTEGRATED HOST-MICROBE METAGENOMICS OF CELL-FREE NUCLEIC ACID FOR SEPSIS DIAGNOSIS Researchers at UCSF and the Chan Zuckerberg Institute have developed a single-sample metagenomic approach combining host transcriptional profiling with unbiased pathogen detection to improve sepsis diagnosis. Sepsis causes 20% of all deaths globally and contributes to 20-50% of hospital deaths in the United States. Early diagnosis and identification of the etiologic pathogen are necessary factors for timely and appropriate antibiotic therapy administration and are critical for sepsis survival. However in over...Thu, 14 Sep 2023 12:00:55 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52419CZB-266Fhttp://czbiohub.technologypublisher.com/technology/52303Thu, 17 Aug 2023 11:30:09 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52303CZB-231Shttp://czbiohub.technologypublisher.com/technology/52302CHEMICALLY MODIFIED BACTERIAL PEPTIDOGLYCAN COMPOSITIONS AND USES THEREOF Researchers at Stanford have developed a new microparticle vaccine scaffold for the development of immunogenic subunit vaccines. Vaccines are one of the most important biomedical advances of the 20th century. Protein vaccines, often referred to as subunit vaccines, have proved to be a particularly useful vaccine strategy. Subunit vaccines have been licensed for the prevention of infectious diseases such as HPV, hepatitis B, and influenza. To assist immunogenicity, subunit vaccines are often administered with adjuvants...Thu, 17 Aug 2023 11:25:49 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52302CZB-230Shttp://czbiohub.technologypublisher.com/technology/52301HIGH-CAPACITY LENTIVIRAL VECTORS Researchers at Stanford have developed new lentiviral vectors that are able to deliver more genetic information than currently available vectors, aiding in the development of gene therapy. Gene therapy is one of the most promising therapeutic avenues in translational medicine. This method involves the genetic engineering of cells in living humans to replace dysfunctional or pathogenic versions of genes. Several methods have been proposed for delivering gene therapy in patients, one of which is lentiviral vectors (LVVs). Lentiviruses are a group of retrovirus...Thu, 17 Aug 2023 11:21:52 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52301CZB-229Shttp://czbiohub.technologypublisher.com/technology/52300Thu, 17 Aug 2023 11:18:59 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52300CZB-226Bhttp://czbiohub.technologypublisher.com/technology/52299MACHINE LEARNING GUIDED DESIGN OF VIRAL VECTOR LIBRARIES Researchers at UC Berkeley have developed a machine learning model that can aide in the design of more efficient viral vector libraries. Directed evolution of biomolecules to generate large numbers of randomized variants is an important innovation in biochemistry. This methodology can be applied to myriad biomolecules of interest, including viruses. In the case of viral variants, this method may be used to select viral variants or viral vectors with specific properties such as tissue type specificity, increased replication capacity, o...Thu, 17 Aug 2023 11:15:13 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/52299CZB-219Bhttp://czbiohub.technologypublisher.com/technology/51784THIN-FILM OPTICAL VOLTAGE SENSOR FOR VOLTAGE SENSING Researchers at UC Berkeley have developed techniques for optical voltage sensing of power grids as well voltage sensing within a human or animal subject. The safe, accurate and economical measurement of time-varying voltages in electric power systems poses a significant challenge. Current systems for measuring power grid voltages typically involve instrument transformers. Although these systems are accurate and robust to environmental conditions, they are bulky, heavy, and expensive, thus limiting their use in microgrids and sensing appli...Thu, 27 Jul 2023 12:53:05 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51784CZB-217Bhttp://czbiohub.technologypublisher.com/technology/51783METHODS RELATED TO AN ALTERNATIVE CONFORMATION OF THE SARS-COV-2 SPIKE PROTEIN Researchers at UC Berkeley have developed methods to probe the conformational landscape of the SARS-CoV-2 Spike protein in the prefusion and ligand binding variations. The Spike protein from SARS-CoV-2 is the primary target for current vaccines against COVID-19 and is the focus of many therapeutic efforts. This large, heavily glycosylated trimeric protein is responsible for mediating cell entry via recognition of host cell receptors. A stabilized prefusion version of the structure of the Spike protein (termed S-2...Thu, 27 Jul 2023 12:44:32 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51783CZB-216Fhttp://czbiohub.technologypublisher.com/technology/51781SINGLE-CELL CULTURE AND SEQUENCING WITH LIPID-MODIFIED OLIGOS Researchers at UCSF have developed methods for sequencing a single cell from a cell culture sample and obtaining morphologic or phenotypic measurements and information by combining sequencing approaches and spatial hashing (e.g. barcoding) at a single cell level. There exists a need in the state of the art for a method of obtaining morphological or phenotypic measurements and information linked with sequence information at the single-cell level. Current spatial transcriptomics technologies require dead tissues and are not compat...Thu, 27 Jul 2023 12:40:06 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51781CZB-213Shttp://czbiohub.technologypublisher.com/technology/51780Thu, 27 Jul 2023 12:34:09 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51780CZB-207Shttp://czbiohub.technologypublisher.com/technology/51478CDK19-SELECTIVE INHIBITORS, AND METHODS OF USE THEREOF Researchers at Stanford have developed compounds that act as potent anti-cancer drugs which selectively inhibit CDK19 over CD8 and promise a greater therapeutic index and reduced systemic toxicity. Breast cancer remains the leading cause of cancer-related death among women worldwide. Patient mortality rates have been decreasing due to earlier detection methods and screening of genetic biomarkers associated with at-risk populations. However, targeted therapeutic strategies have not advanced to an equal extent, in particular against patien...Thu, 06 Jul 2023 13:59:18 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51478CZB-273Shttp://czbiohub.technologypublisher.com/technology/51477LIGHT CONTROLLED PROXIMITY LABELING WITH LOV-TURBO Researchers at Stanford University have developed a new approach for proximity labeling via optogenetic control. Allosteric regulation is common in naturally occurring enzymes in living tissues. However, despite its ability to improve spatial and temporal control, allostery has yet to be used extensively in artificially engineered enzymes. One artificially engineered enzyme, TurboID, achieves broad proximity-dependent biotinylation. While Turbo is useful for mapping interactomes and organelle proteomes both in vivo and in vitro, one impor...Thu, 06 Jul 2023 13:56:58 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51477CZB-223Fhttp://czbiohub.technologypublisher.com/technology/51476PARTICLE-TEMPLATED EMULSIFICATION IN WELL PLATES Researchers at UCSF have developed a new method for emulsifying droplet libraries that is highly scalable. In the past decade, droplet microfluidic technology has revolutionized the sequencing space via single cell RNA-sequencing (scRNA-seq). This technology has vastly increased the resolution of RNA expression in heterogenous tissues and enabled the identification of important molecular signatures and new cell types. Despite their significant contributions to the field, droplet microfluidics have several major limitations that make their imp...Thu, 06 Jul 2023 13:52:45 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51476CZB-222SFhttp://czbiohub.technologypublisher.com/technology/51475METHODS FOR GENERATING NUCLEIC ACID ENCODED PROTEIN LIBRARIES AND USES THEREOF Researchers at UCSF have developed a new method for generating protein libraries encoded with nucleic acids for more efficient identification of molecular binders. Basic biological principles dictate that form follows function. As such, protein-protein binding based on protein form is an essential function for virtually every known biological process. Given that molecular binding of proteins is such an important biological function, it follows that biomedical scientists have developed myriad ways to exploit antib...Thu, 06 Jul 2023 13:45:21 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51475CZB-221Shttp://czbiohub.technologypublisher.com/technology/51474CIRCULAR RNA DERIVED FROM RNA VIRUSES AND RELATED COMPOSITIONS AND METHODS Researchers at Stanford have developed a new therapeutic avenue for the treatment of RNA viral infections. Myriad advances in the field of infectious diseases have been made in the last century, including the development of antibiotics and the widespread use of vaccines. While vaccines offer a powerful tool for prophylaxis against viral (and some bacterial) infections, there is a dearth of treatments available to those who are already infected with a specific virus. Anti-retroviral therapy is a notable exception to t...Thu, 06 Jul 2023 13:39:09 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51474CZB-220Bhttp://czbiohub.technologypublisher.com/technology/51473WOVEN FABRIC BIOELECTRONIC DEVICE Researchers at UC Berkeley and UCSF have developed a bioelectronic device composed of woven fabric to send impulses directly to the brain. While vast scientific advances have been made in the last century in nearly every realm of biomedical science, neuroscience remains one of the last frontiers. While many scientists have made significant headway in terms of our understanding of the underlying mechanisms of brain functions, this progress has yet to result in a plethora of meaningful therapeutic advances. Because of this, many prominent neurological disease...Thu, 06 Jul 2023 13:27:07 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51473CZB-211Fhttp://czbiohub.technologypublisher.com/technology/51472SINGLE-CELL EPIGENOMIC PROFILING USING DROPLET FLUIDICS AND CHEMICALLY MODIFIED HYDROGELS Researchers at UCSF have developed materials and methods for high throughput single cell epigenomic sequencing using a capture/profiling approach with hydrogel scaffolds, thus facilitating a scale and form of epigenomic profiling beyond what has been previously possible. Traditional sequencing methods work with samples of many cells, thereby providing cell signal averages and are unable to resolve cellular heterogeneity. Although many single-cell sequencing methods are now available, there remain seve...Thu, 06 Jul 2023 12:07:51 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51472CZB-209Fhttp://czbiohub.technologypublisher.com/technology/51470Thu, 06 Jul 2023 12:00:32 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51470CZB-208Shttp://czbiohub.technologypublisher.com/technology/51469PROFILING CELL TYPES IN CIRCULATING NUCLEIC ACID LIQUID BIOPSY Researchers at Stanford have developed a method to identify cell types of origin for cell-free RNA from liquid biopsies for a variety of diseases. Cell-free RNA (cfRNA) in blood plasma samples enables dynamic and longitudinal phenotyping for physiological conditions spanning oncology, bone marrow transplants, obstetrics, neurodegeneration and liver disease. Liquid biopsies that measure cfRNA afford broad clinical utility as these transcripts can reflect the health status of multiple tissue types. Most current assays focus on ti...Thu, 06 Jul 2023 11:53:24 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51469CZB-204Shttp://czbiohub.technologypublisher.com/technology/51468GENERATION OF A NEEDLESHAPED BEAM FOR EXTENDED DEPTH OF FOCUS OPTICAL COHERENCE TOMOGRAPHY Researchers at Stanford have developed an innovation that will enhance the depth of the imaging capabilities for optical coherence tomography (OCT) imaging. Optical coherence tomography is a technique for visualizing various medias that was originally developed in the 1990s for the imaging of ocular measurements. This technique is of clinical significance due to its ability to image tissue morphology at a much higher resolution than other clinical imaging techniques such as MRI. OCT provides what are ...Thu, 06 Jul 2023 11:39:29 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51468CZB-203Fhttp://czbiohub.technologypublisher.com/technology/51467METHODS FOR FRAGMENTING COMPLIMENTARY DNA Researchers at UCSF have discovered a new method for fragmenting complimentary DNA for sequencing applications. Sequencing of nucleic acids is one of the most important and transformational methods in biomedical sciences. In recent years, high throughput sequencing has become commonplace in both academic and industry laboratory settings, partially due to its decreasing cost. Most high-throughput sequencing methods require DNA to be fragmented to lengths between 50-250bp. This works well for most sequencing applications given that there are computatio...Thu, 06 Jul 2023 11:32:02 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51467CZB-201Fhttp://czbiohub.technologypublisher.com/technology/51466TESTING DEVICES Researchers at UCSF have developed new methods and a device for at home disease testing. At home testing for SARS-CoV2 using lateral flow technology has been integral to pandemic containment efforts across the globe. Lateral flow diagnostics in general are widely available in point-of-care settings, such as for viral or bacterial infections, or for purchase, such as for pregnancy testing. However, lateral flow assays rely heavily on antigen concentrations in patient samples and are often less accurate than other diagnostic methods. PCR and other direct nucleic acid detection...Thu, 06 Jul 2023 11:24:19 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51466CZB-189Fhttp://czbiohub.technologypublisher.com/technology/51124RECONSTITUTION OF SPLIT-HALOTAG VIA ORTHOGONAL TAG BINDING DOMAINS Researchers at UCSF have developed a method using split HaloTags to tag multiple proteins in a single assay and measure their interactions. Due to new technologies such as CRISPR/Cas9 gene editing, it is now easier to modify DNA than at any other point in human history. One of the most common uses of this revolutionary genomic editing technology is to label proteins by genomic knock-in, specifically fluorescent proteins for identification. However, many fluorescent proteins are relatively large and can interfere with native p...Thu, 18 May 2023 13:30:38 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51124CZB-188Shttp://czbiohub.technologypublisher.com/technology/51123BEAD BASED ASSAY FOR SIMULTANEOUS DETECTION OF BIOMOLECULES Researchers at Stanford University have developed methods for detecting one or more biomolecules within multiple samples in parallel. Rapid and accessible point-of-care diagnostic testing is a cornerstone of public health management of infectious diseases. However, currently available diagnostic tests for viral infections have significant tradeoffs and limitations. PCR is highly sensitive and specific but is slow and expensive, while serology and antigen testing using lateral flow assays are fast and inexpensive, but harbor intrins...Thu, 18 May 2023 13:24:00 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51123CZB-187Fhttp://czbiohub.technologypublisher.com/technology/51122DEVELOPMENT AND VALIDATION OF A 2-GENE HOST-VIRAL TRANSCRIPTOMIC CLASSIFIER FOR ENHANCED COVID-19 DIAGNOSIS Researchers at UCSF and CZ Biohub SF have developed a two gene host signature that detects COVID infection regardless of the causative viral variant. Though COVID-19 has largely transitioned from pandemic to endemic status, viral variants of SARS-CoV2 continue to emerge and enact devastating human health consequences on vulnerable populations. Throughout the pandemic, diagnostic testing has been key to public health mitigation strategies. Direct nucleic acid detection using nasopharyn...Thu, 18 May 2023 13:18:41 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51122CZB-192Shttp://czbiohub.technologypublisher.com/technology/51121DISEASE MANAGEMENT SYSTEM Researchers at CZ Biohub SF and Stanford University have developed a new disease management system that enables efficient population-level tracking of disease incidence rates. In recent years, the risk of pandemics has become much more visible to the public. As a result of ongoing international development and our ever-growing proximity to populations of wild animals, viral zoonotic jumps from animals to humans are becoming more and more common. As we continue to encroach on the natural habitats of many species, including those known to be reservoir species for zoo...Thu, 18 May 2023 13:12:48 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51121CZB-198http://czbiohub.technologypublisher.com/technology/51120LOW COST BEAM-EXPANDING RELAY LENS Researchers at CZ Biohub San Francisco have developed a laser scanning microscope, with significantly reduced complexity and cost, at great benefit to researchers and instrument manufacturers. Optical intervention accompanied with image acquisition of a biological sample using a laser beam scanning microscope is a popular method. Laser beam scanning in 2-dimensional space in a microscope is typically done using two galvanometer scanning mirrors. Currently, to achieve an ideal alignment in which the laser beam focuses on the mirror surface, either a large o...Thu, 18 May 2023 12:57:04 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51120CZB-196Fhttp://czbiohub.technologypublisher.com/technology/51119NUCLEAR DNA-ANTIBODY SEQUENCING FOR JOINT PROFILING OF GENOTYPE AND PROTEIN IN SINGLE NUCLEI Researchers at UCSF have developed a method for joint nucleic acid-protein profiling from single nuclei that can be applied to all manner of biological samples, including frozen tumor specimens or infectious disease samples. Cellular heterogeneity is an aspect that drives disease progression and relapse in cancer, and because cancer cells are heterogeneous in genotype and phenotype, it is especially difficult to link genotypes to immunophenotypes. Single cell analysis thereby provides a powerful too...Thu, 18 May 2023 12:51:57 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51119CZB-193Fhttp://czbiohub.technologypublisher.com/technology/51117METHODS FOR GENERATING PARVALBUMIN-POSITIVE INTERNEURONS Researchers at UCSF and have developed a method for generating an enriched population of parvalbumin-positive interneurons. Parvalbumin-positive interneurons are the largest subpopulation of inhibitory interneurons in the cerebral cortex, and are involved in a variety of neurodevelopmental and neurodegenerative disorders. Based on their important functions in the brain, there is a need in the field for a method to generate parvalbumin-positive interneurons for further study. Stage of Research The inventors have developed a method for...Thu, 18 May 2023 12:38:31 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51117CZB-261Fhttp://czbiohub.technologypublisher.com/technology/51116LOWER RESPIRATORY TRACT INFECTION DIAGNOSTICS Researchers at UCSF, CZ Biohub SF, CZI and University of Colorado have developed a host/pathogen classifier to differentiate patients with lower respiratory tract infections (LRTI) from those with non-infectious acute respiratory illnesses. Lower respiratory tract infections (LRTI) are a leading cause of hospitalizations in the pediatric population. About 12% of children under 5 years old contract a LRTI globally. Due to difficult diagnosis, LRTIs cause more deaths per year than any other type of infection. Early detection of the responsible org...Thu, 18 May 2023 12:29:29 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/51116CZB-185Fhttp://czbiohub.technologypublisher.com/technology/50884MULTIMODE OMICS OF SINGLE TISSUE SAMPLE Researchers at UCSF have developed a method for multimode OMICs analyses of tissue sections using mass spectrometry imaging, spatial transcriptomics, and genomics on a shared sample. Currently, mass spec imaging based on MALDI MS is the best way to show the distribution of small chemicals or metabolites of tissues. However, current techniques require the substrate to be conductive, and thus it cannot be combined with other commercially available gene expression techniques and materials. For instance, measurement of gene expression levels of brain tissu...Thu, 20 Apr 2023 12:05:05 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50884CZB-184Fhttp://czbiohub.technologypublisher.com/technology/50882PARTITIONING CELLS FOR HIGH-THROUGHPUT SINGLE-CELL SEQUENCING Researchers at UCSF have developed a method for high throughput single-cell multi-omic sequencing that is rapid, simple to operate, and offers key innovations over existing technologies. Single-cell sequencing technologies refer to the methods to obtain genomics, transcriptomics or multi-omics information of individual cells. Traditional sequencing methods work with samples of many cells, reveal average signals of the pooled samples, and are thereby unable to resolve cellular heterogeneity. Several single-cell sequencing methods ...Thu, 20 Apr 2023 11:59:10 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50882CZB-182Bhttp://czbiohub.technologypublisher.com/technology/50881ADENO-ASSOCIATED VIRUS VIRIONS AND METHODS OF USE THEREOF Researchers at UC Berkeley And. UCSF have developed a recombinant adeno-associated virus (rAAV) virion, comprising a variant AAV capsid protein, which can exhibit greater infectivity of a macrophage and has potential therapeutic applications. Adeno-associated virus (AAV) belongs to the Parvoviridae family and Dependovirus genus, whose members replicate upon co-infection with a helper virus such as adenovirus. AAV can establish a latent infection in the absence of a helper. The 4.9 kb single-stranded AAV genome is composed of two open ...Thu, 20 Apr 2023 11:52:26 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50881CZB-180Shttp://czbiohub.technologypublisher.com/technology/50880MASSIVELY SCALABLE VIRAL TESTING AND ASYMPTOMATIC SURVEILLANCE Researchers at Stanford have developed a method called Identity Preserving Sample Multiplexing (IPSM) for the scaled up and rapid identification of SARS-CoV-2 positive subjects. At the beginning of the 2020 COVID-19 pandemic, reliably tracking SARS-CoV-2 among patients was a critical step in quarantining, triaging, and treating positive patients. However most hospitals were initially unable to process even 1000 tests per day due to the shortage of reagents and other resources, whereas an order of magnitude or more that number of...Thu, 20 Apr 2023 11:47:08 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50880CZB-179Shttp://czbiohub.technologypublisher.com/technology/50830Thu, 13 Apr 2023 13:20:53 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50830CZB-177Shttp://czbiohub.technologypublisher.com/technology/50828COUPLING-INDEPENDENT, REAL-TIME WIRELESS RESITIVE SENSING THROUGH NONLINEAR PT-SYMMETRY Researchers at Stanford have developed a method for real-time wireless resistive sensing using non-linear PT-symmetry. Fully passive sensors are lower cost and less complex than their active sensor counterparts. Indeed, fully passive sensors consist of two primary components: an inductor and a sensing element. Sensing elements are either a capacitor or a resistor whose value fluctuates in response to a measurable parameter. This measurable parameter is read in fully passive sensors by magnetically coupli...Thu, 13 Apr 2023 13:08:03 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50828CZB-205Bhttp://czbiohub.technologypublisher.com/technology/50627Wed, 15 Mar 2023 16:07:49 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50627CZB-170Shttp://czbiohub.technologypublisher.com/technology/50626Wed, 15 Mar 2023 16:07:12 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50626CZB-169Shttp://czbiohub.technologypublisher.com/technology/50625Wed, 15 Mar 2023 16:06:42 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50625CZB-166Shttp://czbiohub.technologypublisher.com/technology/50624Wed, 15 Mar 2023 16:06:13 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50624CZB-164Shttp://czbiohub.technologypublisher.com/technology/50623Wed, 15 Mar 2023 16:05:25 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50623CZB-165Shttp://czbiohub.technologypublisher.com/technology/50622Wed, 15 Mar 2023 16:05:02 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50622CZB-162Shttp://czbiohub.technologypublisher.com/technology/50621CDK19- SELECTIVE INHIBITORS, AND METHODS OF USE THEREOF Researchers at Stanford University have identified new CDK19-selective inhibitors for the treatment of triple negative breast cancer. Breast cancer remains the leading cause of cancer-related mortality among women worldwide. While early cancer detection methods such as genetic biomarker screening and regular mammograms have significantly reduced breast cancer mortality in resource-rich settings, treatment options have lagged. Triple-negative breast cancer (TNBC) refers to breast cancer that is estrogen receptor (ER), progesterone recep...Wed, 15 Mar 2023 16:03:32 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50621CZB-161Fhttp://czbiohub.technologypublisher.com/technology/50620Wed, 15 Mar 2023 16:02:48 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/50620CZB-224Shttp://czbiohub.technologypublisher.com/technology/48065Mon, 15 Aug 2022 17:44:07 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/48065CZB-206Shttp://czbiohub.technologypublisher.com/technology/48064Mon, 15 Aug 2022 17:43:38 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/48064CZB-195Fhttp://czbiohub.technologypublisher.com/technology/48063Mon, 15 Aug 2022 17:43:09 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/48063CZB-183Fhttp://czbiohub.technologypublisher.com/technology/48035Thu, 11 Aug 2022 13:31:01 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/48035CZB-152http://czbiohub.technologypublisher.com/technology/46852Thu, 19 May 2022 10:43:15 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/46852CZB-151http://czbiohub.technologypublisher.com/technology/46851Thu, 19 May 2022 10:37:04 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/46851CZB-148http://czbiohub.technologypublisher.com/technology/46850Thu, 19 May 2022 10:29:41 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/46850CZB-190Shttp://czbiohub.technologypublisher.com/technology/46632Wed, 11 May 2022 16:26:51 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/46632CZB-146Shttp://czbiohub.technologypublisher.com/technology/46631COMPOSITIONS AND METHODS FOR TREATING RNA VIRUS INFECTION Researchers at Stanford have developed a method of treating or preventing an infection from an RNA virus. Viral pathogens are an ongoing threat to public health worldwide. Upon infection, RNA viruses must remodel host machinery to promote viral protein production while also suppressing host translation to evade host RNA surveillance and antiviral responses. Furthermore, RNA viral genomes are translated from a single open reading frame (ORF) into multidomain polyproteins that require host proteolytic processing and assembly. Dissecting...Wed, 11 May 2022 14:34:49 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/46631CZB-143Fhttp://czbiohub.technologypublisher.com/technology/45729Fri, 14 Jan 2022 14:34:57 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/45729CZB-144Fhttp://czbiohub.technologypublisher.com/technology/45727Fri, 14 Jan 2022 14:22:27 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/45727CZB-140http://czbiohub.technologypublisher.com/technology/45726Fri, 14 Jan 2022 14:19:39 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/45726CZB-138Shttp://czbiohub.technologypublisher.com/technology/45725Fri, 14 Jan 2022 14:17:17 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/45725CZB-137http://czbiohub.technologypublisher.com/technology/45724MADRONE: USER-FRIENDLY BIOINFORMATICS PLATFORM FOR CLUSTER ANALYSIS Researchers at the Chan Zuckerberg Biohub have developed advanced analytics for pathogen infection tracking and control. Health officials use genomic sequence information for both identification and tracking of infectious disease outbreaks. However, most epidemiological analysis of pathogen sequence information in the United States is through rudimentary software tools such as spreadsheets. Tracking of millions of nucleotide sequences using rudimentary tools is cumbersome and error-prone, and limits extracting actionable inf...Fri, 14 Jan 2022 14:13:14 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/45724CZB-136Fhttp://czbiohub.technologypublisher.com/technology/45723AUTOANTIBODIES AS BIOMARKERS FOR AUTOIMMUNE POLYGLANDULAR SYNDROME TYPE 1 Researchers at UCSF and the Chan Zuckerberg Biohub have identified multiple common autoantibody targets in APS1 patients through proteome-wide programmable phage-display. The identification of autoantigens remains a critical challenge for understanding and treating autoimmune diseases. Autoimmune polyglandular syndrome type 1 (APS1) is a rare monogenic form of autoimmunity that presents with widespread T and B cell autoimmune responses to multiple organs. Although APS1 is rare, many autoantibodies in APS1 patients are ...Fri, 14 Jan 2022 14:09:56 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/45723CZB-134Shttp://czbiohub.technologypublisher.com/technology/45722POPTAG: A MODULAR PLATFORM FOR ENGINEERING FUNCTION OF NATURAL AND SYNTHETIC BIOMOLECULAR CONDENSATES Researchers at Stanford have developed a protein, PopTag, that drives phase separation when it is part of a chimeric fusion protein. Biomolecular condensation is a powerful mechanism underlying cellular organization and regulation in cell physiology and disease. Biomolecular condensates can adopt a broad spectrum of material properties, from highly dynamic liquids to semi-fluid gels and solid amyloid aggregates. Perturbing protein condensation and other pathological phase transitions have b...Fri, 14 Jan 2022 14:06:13 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/45722CZB-173Fhttp://czbiohub.technologypublisher.com/technology/45181Tue, 26 Oct 2021 15:20:50 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/45181CZB-133Bhttp://czbiohub.technologypublisher.com/technology/44656Thu, 12 Aug 2021 15:56:22 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/44656CZB-132Bhttp://czbiohub.technologypublisher.com/technology/44655Thu, 12 Aug 2021 15:55:56 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/44655CZB-131http://czbiohub.technologypublisher.com/technology/44654FLASH-MS: FINDING LOW ABUNDANCE SEQUENCES BY CAS9 HYBRIDIZATION MASS SPECTROMETRY Researchers at the Chan Zuckerberg Biohub have developed FLASH-MS, a method for nucleic acid sequence detection by endonuclease digestion and mass spectrometry. The rapid detection of low abundance targets from clinical specimens is a central challenge in clinical diagnostics. The inventors have previously developed a method for enriching sequences of interest in a complex nucleic acid library. Their technique, FLASH (Finding Low Abundance Sequences by Hybridization), uses a sequence-specific nuclease, such as ...Thu, 12 Aug 2021 15:55:39 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/44654CZB-130Shttp://czbiohub.technologypublisher.com/technology/44653METHODS, COMPOSITIONS, AND KITS FOR ASSESSING ENDOMETRIAL TRANSFORMATION Researchers at Stanford and the Chan Zuckerberg Biohub have developed a transcriptomic characterization of human endometrium and identified specific gene signatures for use in evaluating endometrial samples for one or more menstrual cycle events. In a human menstrual cycle, the endometrium undergoes remodeling, shedding and regeneration, all of which are driven by substantial gene expression changes. Traditionally, a cycle can be divided into two major phases by the event of ovulation: the proliferative (preovulatory) ...Thu, 12 Aug 2021 15:55:15 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/44653CZB-202Shttp://czbiohub.technologypublisher.com/technology/44092PREDICTION OF PREECLAMPSIA RISK USING CIRCULATING CELL-FREE RNA Researchers at Stanford and the Chan Zuckerberg Biohub have developed methods for predicting the risk or existence of preeclampsia. Preeclampsia, a multi-organ syndrome diagnosed after 20 weeks of gestation, is associated with an increase in adverse maternal and perinatal outcomes. Detection and diagnosis can prove challenging, as early signs of preeclampsia (PE) can be easily confused with general pregnancy discomfort or other gestational complications. There is an unmet clinical need for high-quality predictive tests for PE ea...Thu, 24 Jun 2021 16:00:38 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/44092CZB-181Fhttp://czbiohub.technologypublisher.com/technology/44091METHODS, COMPOSITIONS AND SYSTEMS FOR PROFILING OR PREDICTING IMMUNE RESPONSE Researchers at Avail Bio, Inc., UCSF and the Chan Zuckerberg Biohub have developed a proteomic technology for capturing the immune response against non-wild type antigens. Immuno-oncologic research demonstrating tumor recognition by the immune system has led to the development of immune checkpoint inhibitors, in which the immune system is stimulated to aggressively target and destroy cancel cells. While ICIs are current standard of care in immuno-oncology, inhibition of immune checkpoints may inadvertently permit a...Thu, 24 Jun 2021 14:59:55 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/44091CZB-163Shttp://czbiohub.technologypublisher.com/technology/44090EMERGENCY USE VENTILATOR Researchers at Stanford have developed a low-cost and rapidly scalable emergency use ventilator for the COVID-19 crisis. The high incidence of COVID-19-related respiratory failure has exposed critical shortages in the supply of mechanical ventilators. Mechanical ventilators, devices that facilitate both oxygenation and ventilation, must reliably deliver a specific volume of gas in a specific time interval. Mechanical ventilators traditionally rely on an internal compressor and mixer to moderate and control the positive-pressure gas mixture delivered to a patient. Ho...Thu, 24 Jun 2021 14:55:38 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/44090CZB-157Fhttp://czbiohub.technologypublisher.com/technology/44089SCITO-SEQ: SINGLE CELL COMBINATORIAL INDEXED CYTOMETRY SEQUENCING Researchers at UCSF have developed SCITO-seq, a new workflow for single cell sequencing-based proteomics. The use of DNA to barcode and tag antibodies has created new opportunities to use sequencing to profile the molecular properties of thousands of cells simultaneously. Furthermore, DNA-barcoded antibodies coupled with advances in microfluidics have enabled droplet-based single cell sequencing (dsc-seq) to profile the surface proteomes of cells. The major limitation of current dsc-seq workflows is the high cost associated w...Thu, 24 Jun 2021 14:52:35 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/44089CZB-175Fhttp://czbiohub.technologypublisher.com/technology/43427BIOSENSOR DETECTION ASSAY FOR ANTI-SARS-COV-2 ANTIBODIES Researchers at UCSF and the Chan Zuckerberg Biohub have developed a serological detection assay for anti-SARS-CoV-2 antibodies. COVID-19, caused by the SARS-CoV-2 virus, has spread throughout the world. Early detection of disease using viral detection assays is critical for containing the spread of this virus. The most widely used tests are PCR-based, which detect viral RNA in patient samples. However, these methods are limited in throughput and take hours or days to produce results. Stage of Research The inventors have developed a...Fri, 16 Apr 2021 12:51:21 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43427CZB-174Fhttp://czbiohub.technologypublisher.com/technology/43426ENGINEERED ACE2 RECEPTOR TRAPS TO BLOCK SARS-COV-2 INFECTION Researchers at UCSF and the Chan Zuckerberg Biohub have developed a set of ACE2 variants which potently block SAR-CoV-2 infection in cells. SARS-CoV-2 is the stain of coronavirus that causes COVID-19. This virus is characterized by spike proteins on its surface, which mediate infection by binding to host cell receptor protein angiotensin-converting enzyme II (ACE2). To date, few antiviral therapeutic agents have demonstrated clinical efficacy in treating COVID-19. Therefore, the is an urgent need for additional pharmaceutical agen...Fri, 16 Apr 2021 12:50:52 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43426CZB-167Fhttp://czbiohub.technologypublisher.com/technology/43425DETECTION ASSAY FOR SARS-COV-2 VIRUS Researchers at UCSF and the Chan Zuckerberg Biohub have developed methods to detect SARS-CoV-2 virus. Clinical laboratory tests and point-of-care tests are needed for screening and diagnosis of individuals infected with SARS-CoV-2 virus. SARS-CoV-2 infection begins with viral Spike protein binding to the human surface receptor protein angiotensin-converting enzyme II (ACE2). As SARS-CoV-2 continues to spread around the world, there is an urgent need for new assay formats to detect viral antigens, such as the surface Spike glycoprotein. Stage of Research ...Fri, 16 Apr 2021 12:50:01 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43425CZB-125Shttp://czbiohub.technologypublisher.com/technology/43423NOVEL UBIQUITOUS CHROMATIN-OPENING ELEMENT FOR STABLE LONG-TERM TRANSGENE EXPRESSION Researchers at Stanford and the Chan Zuckerberg Biohub have identified a novel chromatin remodeling element for stable long-term transgene expression. Gene therapy applications and biomanufacturing of therapeutic proteins require stable long-term transgene expression. Unfortunately, heterologous expression of transgenes in mammalian cells is susceptible to time-dependent epigenetic silencing. Regulatory elements that resist transgene silencing, such as ubiquitous chromatin opening elements (UCOEs), can impro...Fri, 16 Apr 2021 12:48:50 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43423CZB-124Shttp://czbiohub.technologypublisher.com/technology/43012BARCODED CLONAL TRACKING OF GENE TARGETING IN CELLS Researchers at Stanford and the Chan Zuckerberg Biohub have developed a methodology to monitor cell expansion and differentiation following targeted genomic modification. Targeted DNA correction of disease-causing mutations offers promise in the treatment of genetic diseases. Following targeted genomic modification, it is often desirable to follow progeny cells, especially in situations where possible secondary mutations can occur in the genome (e.g., due to off-target effects) such that progeny cells act differently. However, because of t...Thu, 11 Feb 2021 16:49:22 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43012CZB-121Shttp://czbiohub.technologypublisher.com/technology/43011TUNABLE RNA INTERFERENCE VIA NUCLEOTIDE MISMATCH DESIGN Researchers at Stanford, the University of Massachusetts and the Chan Zuckerberg Biohub have developed methods to increase or decrease RNA interference target cleavage rates. Harnessing the power of RNA interference (RNAi), the biological process of RNA-guided and sequence-specific gene expression downregulation, has revolutionized biomedicine by providing means for treating various diseases, especially diseases caused by mutated or aberrant gene expression. However, predicting the specificity and efficiency of RNAi reagents, such as m...Thu, 11 Feb 2021 16:48:26 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43011CZB-123Shttp://czbiohub.technologypublisher.com/technology/43010IMPROVED VARIANTS OF TEV PROTEASE FOR BIOTECHNOLOGICAL APPLICATIONS Researchers at Stanford and the Chan Zuckerberg Biohub have developed methods for producing protease variants with increase catalytic efficiency. Proteases are ubiquitous in biology, and their peptide bond cleavage activities have been harnessed for a wide range of biotechnological applications. TEV (Tobacco Etch Virus) protease is widely used and appealing due to its high sequence specificity for the TEV cleavage site (TEVcs) and orthogonality to mammalian systems. However, a major limitation of TEV protease is its slow ca...Thu, 11 Feb 2021 16:48:18 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43010CZB-120Shttp://czbiohub.technologypublisher.com/technology/43009MULTIPLEXED DETECTION AND CHARACTERIZATION OF MICROBIAL NUCLEIC ACIDS USING SPECTRALLY ENCODED BEADS Researchers at Stanford and the Chan Zuckerberg Biohub have developed a platform for characterizing a population of microbes using spectrally encoded beads. The clinical treatment of microbial infections is dependent on the proper identification and characterization of responsible pathogens. Standard clinical practice for identifying infectious pathogens can require time-consuming culture steps to increase microbial populations before subsequent nucleic acid detection. Other current PCR-based...Thu, 11 Feb 2021 16:47:38 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43009CZB-119Shttp://czbiohub.technologypublisher.com/technology/43008MULTI-PARAMETRIC SINGLE CELL ANALYSIS USING SPECTRALLY ENCODED MICROBEADS Researchers at Stanford and the Chan Zuckerberg Biohub have developed methods for multiple parameter single cell analysis. Methods to analyze single cells are important for evaluating cell-to-cell variability in biological systems, especially where the characteristics of minority cell populations can be masked by bulk population. Single cell analysis methods have traditionally been limited to sequence-based biomolecule quantification (e.g., gene expression analysis via RNA-Seq). There are few technologies that can link...Thu, 11 Feb 2021 16:47:21 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/43008CZB-128Fhttp://czbiohub.technologypublisher.com/technology/42609GENE THERAPY FOR IMMUNO-ONCOLOGY APPLICATIONS Researchers at the Chan Zuckerberg Biohub and UCSF have developed viral gene therapy delivery systems for therapeutic immuno-oncology applications. Glioblastoma is the most common and deadliest primary malignant brain cancer with an overall median survival of 14.6 months. Therapies currently in use, and in development, are limited in safety, efficacy or applicability. Recent advances in immuno-oncology therapies, which harness the power of the immune system to help treat cancer, have resulted in improved patient outcomes in several tumor types. F...Wed, 16 Dec 2020 14:38:19 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42609CZB-114http://czbiohub.technologypublisher.com/technology/42608FLASH (FINDING LOW ABUNDANCE SEQUENCES BY HYBRIDIZATION) Researchers at the Chan Zuckerberg Biohub have developed an improved technique, FLASH, which uses a programmable nuclease to enrich target sequences during preparation of nucleotide libraries. Next generation sequencing (NGS) technology is a powerful tool in diagnostic and functional genomics. However, the detection of low abundance targets from clinical specimens is a central challenge in clinical diagnostics. Current methods for enriching sequences of interest in a complex nucleic acid library can be inefficient, expensive, difficult...Wed, 16 Dec 2020 14:32:18 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42608CZB-111Shttp://czbiohub.technologypublisher.com/technology/42584BROADLY NEUTRALIZING ANTIBODIES AGAINST DENGUE VIRUS Researchers at Stanford and the Chan Zuckerberg Biohub have discovered cross reactive, broadly neutralizing human antibodies against all serotypes of dengue virus. Dengue virus (DENV) is transmitted to humans through mosquitos and causes approximately 400 million global infections each year. DENV has four antigenically distinct serotypes (DENV 1-4), and reinfection with a heterologous DENV serotype is a risk factor for developing potentially fatal severe disease. This increased risk is partially due to the prevalence of cross-reactive ant...Mon, 14 Dec 2020 12:13:53 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42584CZB-139Shttp://czbiohub.technologypublisher.com/technology/42285A METHOD OF IDENTIFYING SMALL MOLECULE INHIBITORS FOR PD-1 Researchers at Stanford, funded in part by the Chan Zuckerberg Biohub, have generated a crystal structure for human PD-1 in complex with one of its ligands, PD-L2, for use in small molecule inhibitor design. Immune checkpoint inhibition of programmed death 1 (PD-1) and its ligand 1 (PD-L1) by monoclonal antibodies (mAbs) has dramatically increased patient survival in many cancer types. While mAbs are clinically beneficial, there is still a need for other small molecule immune checkpoint inhibitors of PD-1. Unfortunately, previous att...Mon, 09 Nov 2020 15:11:56 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42285CZB-129Fhttp://czbiohub.technologypublisher.com/technology/42284ENABLING GENETIC ANALYSIS OF DIVERSE BACTERIA WITH MOBILE-CRISPRi Researchers at UCSF, funded in part by the Chan Zuckerberg Biohub, have generated a modular and effective CRISPRi system for the genetic dissection of non-model bacteria. The vast majority of bacteria, including human pathogens and microbiome species, lack genetic tools needed to associate genes with phenotypes. A method of gene disruption, clustered regularly interspaced short palindromic repeats interference (CRISPRi), enables the robust silencing of target genes by interfering with gene transcription. CRISPRi has demonstrat...Mon, 09 Nov 2020 15:11:49 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42284CZB-113Shttp://czbiohub.technologypublisher.com/technology/42282DISCOVERY OF A HIGHLY THERMOSTABLE CAS9 VARIANT Researchers at Stanford and the Chan Zuckerberg Biohub have discovered a Cas9 protein variant from Ignavibacterium that is thermostable at elevated temperatures. The application of genome editing technologies, such as clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins, has revolutionized molecular biology and biomedicine. CRISPR-Cas9 systems are naturally occurring in many archea and bacteria and function to target and cleave invading nucleic acids. Many Cas9 proteins have been identified, e...Mon, 09 Nov 2020 15:11:32 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42282CZB-110Shttp://czbiohub.technologypublisher.com/technology/42281MULTI-PARAMETRIC AND HIGH-THROUGHPUT SINGLE CELL ANALYSIS Researchers at Stanford, funded in part by the Chan Zuckerberg Biohub, have generated a method for performing multi-parametric and high-throughput single cell genomic and phenotypic analyses. Single cell -omics, the study of diverse biological processes such as genome, RNA or protein expression profiles and the analysis of specific biomolecules at the single cell level has emerged as an important method for understanding biological phenotypes. For example, assaying tumor cells allows determination of mutations enriched in tumor cell p...Mon, 09 Nov 2020 15:11:24 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42281CZB-109Shttp://czbiohub.technologypublisher.com/technology/42280A STRATEGY FOR CREATING VACCINES TO ELICIT ANTIBODIES TARGETING A SPECIFIC EPITOPE Researchers at Stanford, funded in part by the Chan Zuckerberg Biohub, have developed PMD (Protect, Modify, Deprotect), an immunofocusing strategy that can be used in vaccine development for the generation of antibodies targeting a specific epitope. Vaccine development is a ground-breaking accomplishment in the biomedical field, leading to the near eradication of several diseases. Many vaccines provide long-lasting protection against infectious disease by eliciting an antibody immune response against a partic...Mon, 09 Nov 2020 15:11:16 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42280CZB-105Shttp://czbiohub.technologypublisher.com/technology/42276Mon, 09 Nov 2020 15:10:39 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42276CZB-104Shttp://czbiohub.technologypublisher.com/technology/42275AFUCOSYLATED MATERNAL ANTI-DENGUE IGGS ARE A BIOMARKER FOR SUSCEPTIBILITY TO SEVERE DENGUE DISEASE IN INFANTS Researchers at Stanford, funded in part by the Chan Zuckerberg Biohub, have developed a method for determining infant susceptibility to severe dengue disease through measurement of maternal anti-dengue IgG. Mortality rates in severe dengue disease can exceed 20% when patients are not hospitalized but can be reduced to less than 1% with inpatient care. Therefore, the identification of biomarkers for increased susceptibility to dengue disease could dramatically reduce mortality rates ...Mon, 09 Nov 2020 15:10:32 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42275CZB-103Shttp://czbiohub.technologypublisher.com/technology/42274Mon, 09 Nov 2020 15:10:24 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42274CZB-102Shttp://czbiohub.technologypublisher.com/technology/42273METHODS FOR TREATING TRIPLE NEGATIVE BREAST CANCER Researchers at Stanford, funded in part by the Chan Zuckerberg Biohub, have developed a method for treating and predicting treatment efficacy of triple negative breast cancer (TNBC). TNBC is an aggressive subtype of breast cancer that is characterized by a lack of expression of three receptors commonly targeted by breast cancer therapies: estrogen, progesterone and HER2. This cancer affects 20% of all breast cancer patients, yet TNBC currently has no clinically approved targeted therapies. The inventors have now shown that reducing expressi...Mon, 09 Nov 2020 15:10:17 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42273CZB-101Shttp://czbiohub.technologypublisher.com/technology/42225Mon, 02 Nov 2020 14:51:27 GMTBonnevie.Bernardino@czbiohub.orghttp://czbiohub.technologypublisher.com/technology/42225